Bacterial capsule slideshare

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This document discusses capsule staining, which is a technique used to identify the presence of bacterial capsules under a light microscope. It begins by defining bacterial capsules and explaining their functions, which include helping bacteria resist phagocytosis and providing protection. It then discusses the principle of capsule staining, which uses a negative stain to contrast the unstained capsule against stained bacterial cells. The procedure involves smearing a bacterial culture onto a slide with negative stain, staining with a counterstain like crystal violet, and examining under a microscope for unstained capsules surrounding stained cells. Examples of capsule-containing bacteria that can be identified this way include Klebsiella pneumoniae and Bacillus anthracis. Read less. AI-enhanced description.

Bacterial capsule slideshare

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Capsule of bacteria , composition ,function of capsule etc. Read less. Download Now Download to read offline. Recommended Bacterial cell wall. Bacterial cell wall Ashfaq Ahmad. Fimbriae and pili.

Some bacterial cells are surrounded by a viscous substance forming a covering layer or envelope around the cell wall, called a capsule. Bacterial capsules can be visualized by light microscopy using special staining methods. If the layer is too thin to be seen by light microscopy, it is termed a microcapsule. It is called the slime layer if it is so abundant that many cells are embedded in a common matrix. Capsule also known as K antigen is a major virulence factor of bacteria. The principal pathogens that cause pneumonia and meningitis include Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, Klebsiella pneumoniae, Escherichia coli, and group B streptococci have polysaccharide capsules on their surface. Nonencapsulated mutants of these organisms are avirulent. Capsule is a gelatinous layer covering the entire bacterium. In light microscopy, capsules appear to be amorphous gelatinous areas surrounding the cell. Capsule is located immediately exterior to the murein peptidoglycan layer of gram-positive bacteria and the outer membrane Lipopolysaccharide layer of gram-negative bacteria.

Bacterial capsule slideshare

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The bacterial capsule is a large structure common to many bacteria. It is a well-organized layer, not easily washed off, and it can be the cause of various diseases.

When viewed, bacterial capsules appear as a bright halo around the cell on a dark background. Microbial staining shital trivedi. Staining methods. Bohomolets Microbiology Lesson 1 Dr. Cyanobacteria Shraddha Chavan. Dheeraj Kumar. Viewers also liked flagella structure and function. Capsule staining Mohit Hinsu. A drop of serum can be used during smearing to enhance the size of the capsule and make it more easily observed with a typical compound light microscope. Cell wall.