Proc natl acad sci u s a

National Academy of Sciences. Proceedings of the National Academy of Sciences Proc.

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Proc natl acad sci u s a

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands. Abstract A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Substances Antigens Ribosomal Proteins Cellulose.

October 7, View source. Open access.

Abstract : A key component of scientific communication is sufficient information for other researchers in the field to reproduce published findings. For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generated the findings, and any additional information needed such as workflows and input parameters. Many journals are revising author guidelines to include data and code availability. This work evaluates the effectiveness of journal policy that requires the data and code necessary for reproducibility be made available postpublication by the authors upon request. We assess the effectiveness of such a policy by i requesting data and code from authors and ii attempting replication of the published findings.

Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Given the selective value of such repeats, it is likely that several different mechanisms have been responsible for their generation. One mechanism that has been shown to generate relatively long tandem repeats in the kilobase range is rolling circle replication. In this communication, we demonstrate that rolling circle synthesis in a simple enzymatic system can produce tandem repeats of monomers as short as 34 bp. In addition to suggesting possible origins for natural tandem repeats, these observations provide a facile means for constructing libraries of repeated motifs for use in "in vitro evolution" experiments designed to select molecules with defined biological or chemical properties. Abstract Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Publication types Research Support, Non-U. Gov't Research Support, U. Gov't, P.

Proc natl acad sci u s a

Biocompatible gold nanoparticles designed to absorb light at wavelengths of high tissue transparency have been of particular interest for biomedical applications. The ability of such nanoparticles to convert absorbed near-infrared light to heat and induce highly localized hyperthermia has been shown to be highly effective for photothermal cancer therapy, resulting in cell death and tumor remission in a multitude of preclinical animal models. Here we report the initial results of a clinical trial in which laser-excited gold-silica nanoshells GSNs were used in combination with magnetic resonance-ultrasound fusion imaging to focally ablate low-intermediate-grade tumors within the prostate. The overall goal is to provide highly localized regional control of prostate cancer that also results in greatly reduced patient morbidity and improved functional outcomes. This pilot device study reports feasibility and safety data from 16 cases of patients diagnosed with low- or intermediate-risk localized prostate cancer. This treatment protocol appears to be feasible and safe in men with low- or intermediate-risk localized prostate cancer without serious complications or deleterious changes in genitourinary function.

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In other projects. UniProt journal ID. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. Robert L. By using our services, you agree to our use of cookies. IV:5 — via Newspapers. Log in. DOI prefix. BEC editorial. According to Journal Citation Reports , the journal has a impact factor of Finnish Wikipedia.

Signaling through the immune checkpoint programmed cell death protein-1 PD-1 enables tumor progression by dampening antitumor immune responses. Therapeutic blockade of the signaling axis between PD-1 and its ligand programmed cell death ligand-1 PD-L1 with monoclonal antibodies has shown remarkable clinical success in the treatment of cancer. To determine if PDPD-L1-directed immunotherapy could be improved with smaller, nonantibody therapeutics, we used directed evolution by yeast-surface display to engineer the PD-1 ectodomain as a high-affinity pM competitive antagonist of PD-L1.

View source. Retrieved December 12, Proceedings of the National Academy of Sciences Proc. YouTube channel ID. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. July 6, Monroe, Louisiana. Gettysburg, Pennsylvania. According to Journal Citation Reports , the journal has a impact factor of Radi R Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine.