Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time.

The Spectrum Viewer is set to fluorescent mode by default.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin. Compound Name.

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Cart 0. Add a spectrum to begin. Adding Excitation Sources [ Show ] To add one or more excitation sources, click "Excitation Source" in the "Add" submenu on the left part of the screen. A popup box will appear on the top of the page with an input box for the starting wavelength. To view a known dye, scroll down the menu on the left and click to select. Spectra Viewer. Email Required. Download and share fluorochrome spectrum alongside laser and filter configurations Generate, export, and share the final report. Note: if an excitation source is added to the graph, the rightmost column in the information table at the bottom of the page, labeled "Peak Intensity" with the excitation source title in parentheses will show the percentage of the maximum possible intensity for the emission curve of each compound currently on the graph. The emission graph profiles will change in real time as the line is dragged to various places on the horizontal axis, representing the intensity of response to an excitation source at that wavelength. The user may move this excitation source by hovering over the representative vertical line until the cursor changes shape to a symmetrical horizontal arrow; once the cursor has changed shape, left-click and hold while dragging the line to a desired place along the horizontal axis.

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If the desired excitation source is known, click to select. Adjusting Graph Display [ Show ] If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. To change the current selection, click a different dye name. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. The user may then "paste" the link into an email or other form of digital communication by either using the right mouse button or clicking the "CTRL" and "v" keys simultaneously. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters. The light source and filters for the chosen instrument will be displayed automatically. Spectra Viewer and Experiment Design. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. To exit out of the menu, click the small "x" on the upper right corner of the gray bar at the top of the menu. Email Required. A - in a table cell represents no applicable spillover. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu.