3vrm

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These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active TA mutant of Vdh by engineering the putative ferredoxin-binding site. Crystallographic and kinetic analyses indicate that the TA mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25 OH VD3 , a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin-treated Rhodococcus erythropolis cells containing VdhTA. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. A single mutation at the ferredoxin binding site of p vdh enables efficient biocatalytic production of hydroxyvitamin d3.

3vrm

These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active TA mutant of Vdh by engineering the putative ferredoxin-binding site. Crystallographic and kinetic analyses indicate that the TA mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25 OH VD3 , a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin-treated Rhodococcus erythropolis cells containing VdhTA. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. Warning You are using a web browser that we do not support. Our website will not work properly. Please update to a newer version or download a new web browser, such as Chrome or Firefox. Contact us. Data API. This is version 1. A single mutation at the ferredoxin binding site of p vdh enables efficient biocatalytic production of hydroxyvitamin d3. Yasutake, Y. Asymmetric Unit. Macromolecule Content Total Structure Weight:

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3vrm

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Last accounts made up to 30 April We'd also like to use analytics cookies so we can understand how you use our services and to make improvements. It is based in Oxford, England. The company was and is based in London, United Kingdom. RiskLogix RiskLogix provides risk and compliance management solutions. Diligencia primarily serves corporates, financial institutions, government and not-for-profit organizations, and professional services. Drag the structure with the mouse to rotate. The gene-expression cassette encoding Bacillus megaterium glucose dehydrogenase-IV was inserted into the R. Pseudonocardia autotrophica. View PDF Confirmation statement made on 24 March with no updates - link opens in a new window - 3 pages 3 pages. Full crystallographic information is available from OCA. Structural highlights 3vrm is a 1 chain structure with sequence from Pseudonocardia autotrophica.

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Hide this message. Cookies on Companies House services We use some essential cookies to make our services work. It features automated reminders for expiring documents and third-party actions and more. The primary clients are automotive sectors, banks, consumers, retailers, and many more. We'd also like to use analytics cookies so we can understand how you use our services and to make improvements. Drag the structure with the mouse to rotate. Next statement date 24 March due by 7 April Data API. Herein, we describe the creation of a highly active TA mutant of Vdh by engineering the putative ferredoxin-binding site. Next accounts made up to 30 April due by 31 January Crystallographic and kinetic analyses indicate that the TA mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. Cookies on Companies House services We use some essential cookies to make our services work.